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Strong colocalization between xCT and AQP5, as measured by Pearson’s Correlation Coefficient and Manders’ Coefficients, indicated ART’s role in mitigating ferroptosis and restoring salivary secretion capacity ( Figures 7E, F ).ART inhibits KEAP1-mediated ubiquitination and degradation of NRF2, activating NRF2-regulated antioxidant gene expression and rescuing SGECs from ferroptosis

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Validation of the biological and clinical relevance of the identified biomarkers led to the selection of candidate proteins for IHC analysis for computational pathology, which offers a powerful tool for quantifying protein expression and spatial heterogeneity within tumor samples, thereby addressing some of the limitations associated with traditional IHC analysis 41